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recombinant human macrophage colony  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human macrophage colony
    Recombinant Human Macrophage Colony, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+macrophage+colony/Recombinant+Human+M-CSF+Protein/pmc13068806-164-39-45
    Average 96 stars, based on 600 article reviews
    recombinant human macrophage colony - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Refining Evaluation of Bone Mass and Adipose Distribution in Dunnigan Syndrome
    Article Snippet: Then, the quantification of PBMCs was determined and posteriorly magnetic separation (CD14 MicroBeads, MACS, Miltenyi Biotec©, Teterow, Germany) was used to obtain monocytes from which osteoclasts were derived. .. These monocytes were cultured in 3 × 3 wells in 96-well plates with α-MEM containing 10% FBS added sequentially in triplets with 25 ng/mL of human macrophage colony-stimulating factor (MCSF) (R&D systems, catalog 216-MCC, Minneapolis, MN, USA), 25 ng/mL of receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D systems, catalog 390-TN, Minneapolis, MN, USA) and 50 ng/mL of chemerin (R&D systems, catalog 2324-CM, Minneapolis, MN, USA) to stimulate osteoclasts. ..

    Article Title: SARS-CoV-2 ORF8 drives osteoclastogenesis in preexisting immune-mediated inflammatory diseases
    Article Snippet: .. BMDMs were extracted from the femora and tibiae of C57BL/6 mice (The Jackson Laboratory) and cultured in α–minimum essential medium containing 10% FBS, 1% P-S, 1% Glutamax Supplement (Gibco, catalog 35050061), and 25 ng/mL of recombinant human macrophage colony-stimulating factor (R&D Systems, Bio-Techne, catalog 216-MCC) for 6 days. ..

    Article Title: Refining Evaluation of Bone Mass and Adipose Distribution in Dunnigan Syndrome.
    Article Snippet: Then, the quantification of PBMCs was determined and posteriorly magnetic separation (CD14 MicroBeads, MACS, Miltenyi Biotec©, Teterow, Germany) was used to obtain monocytes from which osteoclasts were derived. .. These monocytes were cultured in 3 × 3 wells in 96-well plates with α-MEM containing 10% FBS added sequentially in triplets with 25 ng/mL of human macrophage colony-stimulating factor (MCSF) (R&D systems, catalog 216-MCC, Minneapolis, MN, USA), 25 ng/mL of receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D systems, catalog 390-TN, Minneapolis, MN, USA) and 50 ng/mL of chemerin (R&D systems, catalog 2324-CM, Minneapolis, MN, USA) to stimulate osteoclasts. ..

    Article Title: SARS-CoV-2 ORF8 drives osteoclastogenesis in preexisting immune-mediated inflammatory diseases.
    Article Snippet: .. BMDMs were extracted from the femora and tibiae of C57BL/6 mice (The Jackson Laboratory) and cultured in α–minimum essential medium containing 10% FBS, 1% P-S, 1% Glutamax Supplement (Gibco, catalog 35050061), and 25 ng/mL of recombinant human macrophage colony-stimulating factor (R&D Systems, Bio-Techne, catalog 216-MCC) for 6 days. ..

    Recombinant:

    Article Title: SARS-CoV-2 ORF8 drives osteoclastogenesis in preexisting immune-mediated inflammatory diseases
    Article Snippet: .. BMDMs were extracted from the femora and tibiae of C57BL/6 mice (The Jackson Laboratory) and cultured in α–minimum essential medium containing 10% FBS, 1% P-S, 1% Glutamax Supplement (Gibco, catalog 35050061), and 25 ng/mL of recombinant human macrophage colony-stimulating factor (R&D Systems, Bio-Techne, catalog 216-MCC) for 6 days. ..

    Article Title: Artemisinin derivative SM934 targets α-enolase to inhibit PEP-mediated TAK1 stabilization and inflammation.
    Article Snippet: 1State Key Laboratory of Immune Response and Immunotherapy, Department of Health Management Center of the First Affiliated Hospital, Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China 2Institute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei 230032, China 3Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 4Laboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

    Article Title: Engineering osteoclast resorption units via sacrificial microgels in a bone-on-chip platform
    Article Snippet: PBMCs were then incubated in BD IMagTM anti-human CD14 magnetic particles (BD-Biosciences) and magnetically separated according to the manufacturer's instructions. .. CD14 + cells were seeded in T75 flasks in α-MEM (Gibco) supplemented with 10% FBS (Sigma-Aldrich), 1% penicillin/streptomycin (Gibco) (Complete Medium) and 25 ng mL −1 of recombinant human macrophage colony stimulating factor (rhM-CSF, R&D Systems) at 5% CO 2 at 37 °C in a humidified incubator. .. After 2 days, the media were changed to α-MEM supplemented with 10% FBS, 1% penicillin/streptomycin, 25 ng mL −1 rhM-CSF and 25 ng mL −1 recombinant human receptor activator of nuclear factor κB ligand (rhRANKL, R&D Systems) and cells were cultured for up to seven days, changing media twice.

    Article Title: SARS-CoV-2 ORF8 drives osteoclastogenesis in preexisting immune-mediated inflammatory diseases.
    Article Snippet: .. BMDMs were extracted from the femora and tibiae of C57BL/6 mice (The Jackson Laboratory) and cultured in α–minimum essential medium containing 10% FBS, 1% P-S, 1% Glutamax Supplement (Gibco, catalog 35050061), and 25 ng/mL of recombinant human macrophage colony-stimulating factor (R&D Systems, Bio-Techne, catalog 216-MCC) for 6 days. ..



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